DOT Compliance Focused

Learn the federal procedures, collector responsibilities, and documentation requirements required for DOT oral fluid drug testing.

Hands On Certification Pathway

Progress from interactive online learning to a live Mock Collection Evaluation designed to verify competency before certification.

Built for Real World Collection Professionals

Designed for collectors, collection sites, occupational health providers, TPAs, mobile collectors, and employers seeking standardized, professional training.

About the course

The DOT Oral Fluid Specimen Collector Training provides comprehensive instruction on the federally regulated oral fluid collection process using the Quantisal™ II Oral Fluid Collection Device. Throughout this course, you'll learn how to prepare the collection site, verify donor identification, perform compliant specimen collections, complete the Federal Custody and Control Form (CCF), maintain chain of custody, and respond appropriately to common collection scenarios. The course combines instructional videos, knowledge assessments, and a live Mock Collection Evaluation to help ensure collectors are prepared to perform oral fluid collections confidently and in compliance with applicable DOT regulations.

Meet Your Instructor

This training has been developed by FluidPath Academy™, combining regulatory guidance, industry best practices, and practical operational experience to prepare collectors for professional DOT oral fluid specimen collection. The curriculum is designed to promote procedural accuracy, specimen integrity, and consistent application of federal collection standards while supporting workforce readiness across the transportation industry.

Curriculum

17 Chapters · 54 Lessons

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      Course Terms of Use

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      LESSON 0.1 — Welcome to DOT Oral Fluid Collector Training

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      LESSON 0.1 — Knowledge Check

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      LESSON 1.1 — Role of the Oral Fluid Collector

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      LESSON 1.1 — Knowledge Check

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      LESSON 1.2 — Who Can and Cannot Be a Collector

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      LESSON 1.2 — Knowledge Check

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      LESSON 1.3 — DOT Qualification Training Requirements

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      LESSON 1.3 — Knowledge Check

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      LESSON 1.3A — Who Can Conduct DOT Oral Fluid Collector Training?

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      LESSON 1.3A — Knowledge Check

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      LESSON 1.4 — Refresher Training & Documentation

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      LESSON 1.4 — Knowledge Check

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      LESSON 1.4A — Paper CCF vs Electronic CCF Training Requirements

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      LESSON 1.4A — Knowledge Check

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      LESSON 2 — The Collection Site

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      LESSON 2 — Knowledge Check

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      LESSON 3 — Securing a Collection Site

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      LESSON 3 — Knowledge Check

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      LESSON 4.1 — Collection Supplies

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      LESSON 4.1 — Knowledge Check

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      LESSON 4.2 — Getting to know the Quantisal II device

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      LESSON 4.2 — Knowledge Check

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      LESSON 5.1 — Introduction to Federal Custody Form (CCF)

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      LESSON 5.2 — Physical Completion Of The Chain Of Custody Form (CCF)

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      LESSON 5 — Knowledge Check

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      LESSON 6 — Information Employers Must Provide to Collectors

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      LESSON 6 — Knowledge Check

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      LESSON 7 — Identifying the Employee

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      LESSON 7 — Knowledge Check

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      LESSON 8.1 — Preparing for the Collection

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      LESSON 8.2 — Donor Preparation & Oral Cavity Inspection

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      LESSON 8.3 — The 10-Minute Wait Period

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      LESSON 8.4 — Device Selection & Expiration Verification

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      LESSON 8.5 — Hand Cleaning & Beginning Collection

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      LESSON 8.6 — Collecting & Inspecting the Specimen Using the Quantisal II Oral Fluid Collection Device

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      LESSON 8.7 — Splitting & Sealing the Specimen

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      LESSON 8.8 — Donor Step 5 & Collector Step 4

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      LESSON 8.9 — Packaging & Shipping

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      LESSON 8 — Knowledge Check

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      LESSON 9 — Insufficient Oral Fluid Specimen Procedures

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      LESSON 9 — Knowledge Check

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      LESSON 10 — Refusal to Test

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      LESSON 10 — Knowledge Check

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      LESSON 11 — Unusual Collections

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      LESSON 11 — Knowledge Check

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      LESSON 12 — Correcting Collection Problems

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      LESSON 12 — Knowledge Check

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      LESSON 13 — Record Maintenance

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      LESSON 13 — Knowledge Check

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      LESSON 14 — Questions and Answers

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      Final Certification Assessment

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      Schedule Your Mock Collection

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      Assignment: Upload Your 5 Mock Collection Scenarios (6 CCFs Total)

Start Your Certification Journey

Enroll today to begin your DOT Oral Fluid Specimen Collector Training and take the first step toward becoming a qualified oral fluid specimen collector.

$149.00

Enroll now